Transformation of Tomato with TYLCV Gene Silencing Construct Using Optimized Agrobacterium-Mediated Protocol
نویسندگان
چکیده
منابع مشابه
Agrobacterium-Mediated Transformation of Pomegranate (Punica granatum L.) ‘Yousef Khani’ Using the gus Reporter Gene
In this study, an efficient Agrobacterium-mediated transformation method was developed forpomegranate (Punica granatum L.), a difficult-to-transform plant. In vitro shoot segments wereinoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vectorpBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....
متن کاملGenetic Manipulation of Tomato (lycopersicon Esculentum) Using Wga Gene through Agrobacterium Mediated Transformation
The major problems faced in the field of Agriculture are loss in crop yield caused by insects, Herbs (weeds), viruses and the pathogens and the pests associated loss is about 14% of total agricultural production. The use of pesticides has resulted in adverse effect on the beneficial organisms and other plant parts such as leaves, fruits and has reached pollution levels, which has become a major...
متن کاملAgrobacterium-mediated transformation of tomato (Solanum lycopersicum L.) using the expansin 10 (CsEXP10) gene.
The cucumber expansin 10 (CsEXP10) gene was previously cloned from young cucumber fruits but its role has not been defined. To determine the role of this gene in plant growth and development, a CsEXP10 gene transformation system was established. The open reading frame of the gene was inserted behind the CaMV35S promoter of vector pCAMBIA1301, and the construct was introduced into tomato plants ...
متن کاملThe Optimized Method of Agrobacterium Mediated Transformation in Nitraria schoberi (Ghar-e-Dagh in Persian)
Ghar-e-Dagh (Nitraria schoberi) is a native perennial plant profoundly resistant to salinity and drought. This plant, having a well-developed deep rooting system can act as sand dunes stabilization agent and also, due to its special alkaloids may perform a very significant role in pharmaceutical industries. Considering the importance of genetic engineering as a novel method to generate...
متن کاملOptimization of gene transfer with Agrobacterium to Bonab tomato cultivar using chitinase gene
Tomato is an important economic crop with nutritional and scientific value for study. Due to its agronomic importance and sensitivity to biotic and abiotic stresses, using new methods in breeding this crop is necessary. In this study, the effects of some factors, such as pre-cultivation time (1, 2, 3 and 4 days), Agrobacterium concentration (OD600nm = 0.4, 0.6, 0.8, and 1), inoculation time (5,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology(Faisalabad)
سال: 2008
ISSN: 1682-296X
DOI: 10.3923/biotech.2008.537.543